The three most common read modes in a single-mode microplate reader from Molecular Devices are absorbance, fluorescence, and luminescence. The absorbance application notes include cell viability, cytotoxicity, cell proliferation, DNA/RNA quantitation, enzyme kinetics, bacterial / microbial growth, endotoxin assays, ELISA / immunoassays, protein quantitation and many more.
Luminescence plate readers from MOLECULAR DEVICES offer greater sensitivity than absorbance or fluorescence detection and they also are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester assays. Luminescence assays with long-lived signal (glow) can be detected by any luminescence reader, while flash assays with short-lived signal (flash) require a reader equipped with injectors.
Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. There are often fluorescent alternatives for applications like cell viability and nucleic acid quantification.
Multi-mode plate readers from MOLECULAR DEVICES provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Some technologies such as imaging, alsphascreen or western blot detection can be added to these multi-mode plate readers.