Measure fluorophore molecules emitted fluorescence
Fluorescence spectrometers are used to excite fluorophore molecules and measure its emitted fluorescence. The spectrometer introduces ultra-violet or visible light using a photon source, like a laser, a xenon lamp or LEDs. The spectrometer focuses the monochromatic wavelength towards the sample then the sample emits a wavelength, which travels to the detector which is usually set at a 90-degree angle to the light source to avoid any interference from the transmitted excitation light. Photons emitted hit a photo detector after that a computer software connected to the detector generates a spectrum, a graphical representation that shows which wavelengths the sample absorbs.