Home » Products » Separation & NIR Solutions » Separation Instruments » Buchi Preparative Chromatography Solutions
Preparative Chromatography Solutions
|
Performance and flexibility fitting your needs
Buchi Preparative Chromatography Solutions
Buchi’s preparative chromatography solutions are engineered to provide a comprehensive platform for the isolation and purification of chemical compounds, ranging from small organic molecules to complex natural products. The technical architecture of these systems allows for the integration of both Flash chromatography and Preparative HPLC (High-Performance Liquid Chromatography) within a single system, offering researchers the flexibility to switch between low-pressure and high-pressure methods depending on the required purity and sample complexity.
The efficiency of the purification process is further enhanced by advanced FlashPure cartridges, which are offered in a wide range of sizes and geometries to suit specific stationary phase requirements. These prefilled cartridges cover various particle sizes, enabling users to optimize the compromise between separating power (resolution) and the amount of sample that can be purified (loading). By providing tools that facilitate the optimization of parameters at a small scale before adaptation to larger industrial sizes, Buchi ensures a seamless scale-up process.
Integration of Flash and Prep HPLC Technology
The core technical advantage of these solutions is the ability to combine Flash and Prep HPLC on a single instrument. This integration allows chemists to perform rapid initial purifications using Flash chromatography and then transition to high-resolution Prep HPLC for final polishing of the compound. By supporting both techniques, the system handles a wide pressure range and diverse column types, providing the necessary versatility to process samples of varying complexity and concentration on a unified platform.
High-Performance Stationary Phase Geometries
Buchi utilizes specialized prefilled cartridges that cover a broad spectrum of stationary phases and particle sizes. These cartridges are designed with specific geometries to ensure uniform flow distribution and minimize band broadening during the elution process. The variety of sizes enables the user to choose the cartridge that best suits their purification needs, ensuring that the stationary phase chemistry is perfectly aligned with the target analyte's polarity and chemical properties for optimal separation.
Separation of Complex Natural Products
In the field of natural product research, such as cannabinoid separation, the instruments are designed to address the challenge of purifying medically active substances from samples containing more than one hundred identified compounds. Many of these compounds share similar chemical structures, making baseline resolution difficult to achieve. The systems are optimized to isolate specific peaks even without baseline resolution, allowing for high-purity recovery of substances used for pain relief and other pharmaceutical applications.
Technical Scale-Up and Loading Optimization
The transition from laboratory-scale research to industrial-scale production is a critical factor in chromatography. Buchi's systems facilitate this scale-up process by allowing researchers to optimize separation parameters at a small scale first. Since increasing the loading of a sample usually decreases separation efficiency, the technical design of the instruments ensures that resolution is maintained as much as possible when moving to larger columns and higher flow rates, ensuring consistency in product quality.
Advanced Solvent and Flow Management
To ensure reproducible results, the systems incorporate sophisticated flow management and gradient control. Precise pump technology delivers accurate solvent mixtures, which is essential for maintaining the stability of the mobile phase throughout the separation. This level of control is necessary for complex gradient elutions where subtle changes in solvent concentration are required to separate closely eluting peaks, directly impacting the final purity and yield of the isolated compound.
Versatility Across Pharmaceutical Applications
The chromatography solutions are engineered to handle the rigorous demands of pharmaceutical purification. This includes the isolation of active pharmaceutical ingredients (APIs), the removal of impurities, and the purification of intermediates. The systems provide the high pressure required for HPLC and the high flow rates needed for Flash, making them suitable for everything from early-stage drug discovery to the production of high-value compounds in regulated environments.
Software Control and Parameter Optimization
The instruments are controlled by intelligent software that manages the entire purification sequence. This software allows for the real-time monitoring of chromatograms and the automated collection of fractions based on detector signals. Technical parameters such as flow rate, solvent composition, and detection wavelengths can be precisely adjusted. This automation reduces manual error and allows for the implementation of complex separation strategies that maximize the recovery of the target analyte.
Efficiency in Multi-Component Sample Processing
When dealing with multi-component samples, a compromise must often be found between separating power and the total amount purified. Buchi's technical design addresses this by offering a range of resolution options. For industrial-scale applications, the systems emphasize larger capacity and loading capabilities while maintaining enough resolution to separate the most important components. This focus on efficiency ensures that high-purity fractions are obtained in the shortest time possible, reducing overall process costs.
Read More About Buchi Products Here.
