Compact Integrated System Design

The technical hallmark of the Buchi Pure platform is its compact integration. Unlike traditional modular systems that occupy significant bench space, this system consolidates the pump, detector, and fraction collector into a small footprint. Technically, this reduces the internal tubing volume, which is essential for maintaining peak resolution and minimizing sample dispersion. The integrated design also simplifies system maintenance and ensures that all components are electronically synchronized for precise purification runs.

Dual Flash and Preparative HPLC Capability

A major technical advantage of this system is its versatility in handling both Flash chromatography and Preparative HPLC. Technically, the high-pressure pumps are engineered to deliver stable flow across a broad range, allowing the user to switch between rapid silica-based Flash purification and high-resolution Prep HPLC using the same instrument. This flexibility is vital for laboratories that transition frequently between crude purifications and final compound isolation.

Advanced Safety and Environmental Protection

Safety is integrated into the technical core of the system through automated monitoring features. The instrument includes specialized leak sensors that automatically shut down the pump if a solvent breach is detected. Furthermore, the system is technically designed for closed-loop operation, featuring a pressurized solvent sensing system that prevents the air from entering the pumps and reduces the emission of volatile organic compounds (VOCs) into the laboratory atmosphere.

High-Performance Universal Detection (ELSD)

For compounds that lack chromophores and cannot be detected by UV, the system can be technically equipped with an Evaporative Light Scattering Detector (ELSD). Technically, ELSD provides universal detection by nebulizing the eluent and measuring the light scattered by the remaining non-volatile analyte particles. This capability is essential for purifying carbohydrates, lipids, and other non-UV active compounds, ensuring that no fraction is missed during the purification process.

Ease of Use with Smart Software Interface

The technical complexity of purification is managed through a highly intuitive software interface. The software allows for real-time monitoring of the chromatogram and provides the technical capability to modify gradients on-the-fly. This allows the operator to respond immediately to co-eluting peaks or unexpected sample behavior. The system also features automated methods for cleaning and column conditioning, reducing manual intervention and increasing laboratory efficiency.

Efficient Fraction Collection and Management

The integrated fraction collector is technically designed for high precision and flexibility. It supports a variety of rack sizes and formats, ensuring that purified compounds are collected in the most suitable containers. Technically, the software controls the collector based on time, volume, or detector signal (peak-based collection). This ensures that the purified analyte is isolated with maximum purity and minimal dilution, facilitating downstream analysis and drying.

Robust Fluidics for Reliable Performance

The fluidic system is technically engineered with high-quality materials to resist a wide range of organic solvents and buffers. The pumps utilize a multi-piston design to ensure a pulse-free flow, which is critical for maintaining column stability and ensuring reproducible retention times. Technically, the high-pressure tolerance of the system allows for the use of small-particle columns, enabling faster separations and improved resolution for difficult-to-purify mixtures.

Sample Injection Flexibility

To accommodate different sample types and volumes, the system offers various injection modes. Technically, it supports both liquid injection for soluble compounds and solid-loading for samples with limited solubility. The solid loading system ensures that the sample is introduced onto the column as a narrow band, which is technically necessary to prevent peak broadening and ensure the highest possible purification efficiency for large-scale samples.

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